| 1. | Detecting gp of hfrs virus with double antibodies sandwich elisa
法检测肾综合征出血热汉滩病毒糖膜蛋白 |
| 2. | Sandwich elisa assay is a sensitive , specific and stable technique
巩乃a法检测可溶性比卜工灵敏、特异、稳定； 2 |
| 3. | Preparation of monoclonal antibodies against troponin i and development of one - step sandwich elisa
单克隆抗体制备及酶免疫法的建立 |
| 4. | Circulating antigen detection in schistosomiasis japonica patients by sandwich elisa using polyclonal and monoclonal antibodies
法检测日本血吸虫病患者血清循环抗原 |
| 5. | Sandwich elisa is an immunoassay technique in common use , especially for the determination of macromolecular protein antigen
双抗体夹心elisa是常用的免疫分析技术，主要适用于检测大分子的蛋白质抗原。 |
| 6. | Cells were collected after being passaged 5 times and used to identify infection by rt - pcr , direct fluorescent antibody , sandwich elisa . results indicated that integrated particles of csfv could be obtained from constructed full - length cdna
连续传代5代以后，收集各w摘要代细胞至第10代，采用rt一pcr 、直接荧光抗体染色和夹心elisa技术进行鉴定，结果均为阳性。 |
| 7. | Methods : sandwich elisa assay was used , w6 / 32 mcab serving as solid phase antibody and 3 2m antibody as the first antibody . the second antibody - hrp conjugate was added for coloration . standard curve was obtained by shla - i standard reagent in serial dilution . the amount of shla - i in the samples was determined : 1
方法：以w6 32包被酶标板，捕捉样品中可溶性hla ，加入一抗2m抗体，再加酶标二抗及底物显色。根据可溶性hla -的不同浓度标准品显色后的od值绘制标准曲线： 1 |
| 8. | Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides , we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library , which may be another form of antagonist for tnfa , and the mimotopes were identified by sandwich elisa . after 3 rounds of screening , we got 9 phage clones identified as positive clones which can bind with mcab j1d9 . we also identified the binding between mimotopes and tnf receptor by competitive elisa , and the results showed strongly binding . the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c
在对噬菌体环七肽库进行三轮亲和性筛选后，随机挑选20个噬菌体克隆， elisa鉴定出9个阳性克隆，经dna测序推出三种氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中优势克隆序列为c - rrpaqsg - c ；鉴定结果显示阳性克隆能够与tnf受体结合，并且能够阻断tnf与受体的结合，提示筛选得到的环七肽克隆展示肽具有tnf的抗原性及与tnf受体结第一军医大学顾士学位论文合的特性，为tnfa表位模拟肽。 |
| 9. | Our research can be divided into the following four parts : part i screening of tnfa - binding peptide from phage display peptide library : the tnfa binding - peptides were screened from c7c phage display peptide library by using rhtnfa as target protein and identified by sandwich elisa , for exploring whether the binding - peptides can be used as antagonist of tnfa or not
筛选tnf小分子模拟肽及结合肽对于tnf研究具有重要的意义。本研究包括以下四个方面： 1 、 tnf结合肽的研究：以rhtnf为靶对噬菌体环七肽库进行筛选，以寻求可拮抗tnf活性的小分子短肽。 |
